In conjunction with the 96-throughput full-automatic Nucleic Acid Extraction instrument, the reagent kit serves to extract genomic DNA/RNA of pathogenic microorganisms from serum or plasma, cultured cells, saliva, alveolar lavage fluid, nasopharyngeal aspirates, and swab samples. The reagent kit is provided with the magnetic bead and buffer solution system featuring unique separation functions, with the special chemical group on the magnetic bead surface exerting extremely strong enrichment forces to DNA/RNA under given conditions for reversible release of DNA/RNA when conditions change, so as to separate and purify DNA/RNA as soon as possible and minimize impurities such as the protein. The DNA/RNA extracted by this kit can be widely used in various downstream Molecular Biology experiments, such as sequencing, genetic typing and qPCR.
[Product name]
Nucleic acid extraction kit [Packing Specification] 96 Reactions
[Intended use]
It is used for nucleic acid extraction, enrichment, purification and other steps. The processed product may be used for clinical in vitro testing.
[Transportation condition]
Transportation under room temperature
[Preservation condition]
Preservation under room temperature, or long-term preservation at 2-8 °C [Period of validity] 12 months
[Applicable Instrument]
Techstar YC796 automatic nucleic acid extraction system, Thermo Scientific KingFisher Flex and other 96-throughput automatic nucleic acid extractor.
Notice: Please read the instruction of this product carefully and follow the operation instructions. Appropriate protective glasses, clothes and gloves are required during operation.
In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, molecular biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.
[Precautions]
1.As a disposable product, this product cannot be reused.
2.Preservation of the reagent plate or reagent in low-temperature places such as the refrigerator may lead to relatively high solution viscosity or sediments. For such sediments found before operation, please heat the reagent plate or reagent at 37 °C and mix it gently to dissolve the sediments. Bubbles must be prevented during mixing.
3.Repeated freezing and thawing of the reagent plate are forbidden, otherwise the magnetic bead may be damaged.
4.If the aluminum film breaks during the process of tearing, rotate the reagent plate at an angle and tear it in a new direction.
5.Please operate carefully, as the solution contains a guanidine salt protein denaturant, which is corrosive. If it accidentally
splashes on the skin, please rinse with plenty of water.
6.The reagent plate must be used within 30 minutes after unsealing, as long-term placement may affect the extraction effect;
7.To prevent reduced activity of proteinase K, sample is added to the sample plate before addition of proteinase K.
8.Each eluent hole is preloaded with a volume of 100 μL. A larger elution volume requires supplement of a certain volume of
TE in accordance with the instrument.
9. Fresh sample extraction is recommended. Repeated freezing and thawing of samples may lead to significantly decreased quantity of the nucleic acid.
10. As RNA is easily degradable, please use Rnase-free consumables. The nucleic acid must be used shortly after extraction and purification. If not, please store at minus 20 degrees Celsius. Long-term storage is recommended below minus 70 degrees Celsius.
Company Introduction
Found in 2009,Wuxi NEST Biotechnology is a worldwide manufacturer of plastic laboratory products. State-of-the-art manufacturing equipment is used on an expansive manufacturing site based in Wuxi, China. Decades of plastic-injection molding experience allows us to maintain the highest quality and performance standards expected by the hospitals, research institutions and indusrial/clinical laboraories that we serve, NEST is your best choice for laboratory consumables.
NEST has launched over 200 plastic consumable products for cell culture, molecular bislogy, immunoassays, liquid handling and storage. With stricty controlled ISO 8 grade clean rooms, exacting manufacturing standards and rigid QA procedures, the NEST brand enjoys an excellent reputation nationwide and abroad. NEST products have been exported to North America, Europe, Japan, Korea, India and other countries.
NEST is committed to innovation. We continue to research and develop new platic consumables suitable for life sciences research so that we can provide more cost-effective solutions and cutting-edge laboratory consumables for our end-users.
NEST has continually positioned itself as a brand for high end laboratory consumables.
Our Services & Strength
NEST boasts of a high power laminar flow clean room meeting the ISO 8 grade with a floor space of 2,000 square meters. These facilities are equipped with a central feeding system, high precision injection machinery from Japan, robot arms with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment machines from Belgium, Germany and USA. Inner room moisture content and temperature are adjustable. In 2013, NEST installed a Rhodotron TT 200 from Belgium IBA, a world leader in the E-Beam sterilization industry. NEST completes a full process chain from the sourcing of the raw materials to the in-house sterilizationof the final products ready for delivery to clients.
NEST’s manufacturing site has been certified with an ISO 9001:2008 management system. Our sterilization procedures meet the ISO 11137:2006 and ISO 13485 standards.
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