NEST Cell Culture Inserts With ISO9001&13485 certified, NEST Cell Culture Inserts are manufactured in 100,000 grade cleaning room with USP 6 grade raw materials. Nest is committed to be your best choice for laboratory and medical consumables.
Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.
Application：
Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent andindependent cell lines.
This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry outmetabolic activities in a more natural manner because the cells feed both apically and basolaterally.
Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.
Product Features：
·Innovative hanging design facilitates pipetting.
·Supplied in multiple well plates: 6-well, 12-well, 24well.
·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
·Compatible with most solvents used for fixing and dyeing.
| | Packing specification | Inserts/plate | Insert Diameter(mm) | Well Volume(ml) | Insert Volume(ml) | Insert Membrane Growth Area（cm²） | | 6 | 6 inserts/6well | 24 | 2.6 | 1.5 | 4.67 | | 12 | 12 inserts/6well | 12 | 1.5 | 0.5 | 1.12 | | 24 | 12 inserts/6well | 6.5 | 0.6 | 0.1 | 0.33 | | 100mm dish | 1 inserts/6well | 75 | 13 | 9 | 44 | | Pore Size(um) | Membrane Density（well/cm²） | | 0.4 | 1x10^8 | | 3.0 | 2x10^6 | | 5.0 | 4x10^5 | | 8.0 | 1x10^5 | Cell Culture Insert, PC Membrane Individually packaged in peelable Tyvek film and Blister, sterile. | TC Treated Cat.NO. | Non-Treated Cat.NO. | Description | Pore Size (μm) | Membrane Transparence | Insert Packing | Plate Packing | | 723001 | 723011 | 6 Cell Culture Inserts + 6 Well Plate | 3.0 | Translucent | 6 | 24 | | 724001 | 724011 | 12 Cell Culture Inserts + 12 Well Plate | 3.0 | Translucent | 12 | 48 | | 725001 | 725011 | 12 Cell Culture Inserts + 24 Well Plate | 3.0 | Translucent | 12 | 48 | | 723101 | 723111 | 6 Cell Culture Inserts + 6 Well Plate | 0.4 | Opaque | 6 | 24 | | 724101 | 724111 | 12 Cell Culture Inserts + 12 Well Plate | 0.4 | Opaque | 12 | 48 | | 725101 | 725111 | 12 Cell Culture Inserts + 24 Well Plate | 0.4 | Opaque | 12 | 48 | | 724201 | 724211 | 12 Cell Culture Inserts + 12 Well Plate | 5.0 | Translucent | 12 | 48 | | 725201 | 725211 | 12 Cell Culture Inserts + 24 Well Plate | 5.0 | Translucent | 12 | 48 | | 723301 | 723311 | 6 Cell Culture Inserts + 6 Well Plate | 8.0 | Translucent | 6 | 24 | | 724301 | 724311 | 12 Cell Culture Inserts + 12 Well Plate | 8.0 | Translucent | 12 | 48 | | 725301 | 725311 | 12 Cell Culture Inserts + 24 Well Plate | 8.0 | Translucent | 12 | 48 | | 726001 | / | 100 mm Cell Culture Insert-Dish | 3.0 | Translucent | 1 | 10 | Cell Culture Insert, PET Membrane Individually packaged in peelable Tyvek film and Blister, sterile. | TC Treated Cat.NO. | Non-Treated Cat.NO. | Description | Pore Size (μm) | Membrane Transparence | Insert Packing | Plate Packing | | 723121 | 723131 | 6 Cell Culture Inserts + 6 Well Plate | 0.4 | Opaque | 6 | 24 | | 724121 | 724131 | 12 Cell Culture Inserts + 12 Well Plate | 0.4 | Opaque | 12 | 48 | | 725121 | 725131 | 12 Cell Culture Inserts + 24 Well Plate | 0.4 | Opaque | 12 | 48 | | 723021 | 723031 | 6 Cell Culture Inserts + 6 Well Plate | 3.0 | | 6 | 24 | | 724021 | 724031 | 12 Cell Culture Inserts + 12 Well Plate | 3.0 | | 12 | 48 | | 725021 | 725031 | 12 Cell Culture Inserts + 24 Well Plate | 3.0 | Translucent | 12 | 48 | |
New addition: 1μm & 8μm PET Membrane
| TC Treated Cat.NO. | Non-Treated Cat.NO. | Description | Pore Size (μm) | Membrane Transparence | Insert Packing | Plate Packing |
| 723421 | 723431 | 6 Cell Culture Inserts + 6 Well Plate | 1.0 | Opaque | 6 | 24 |
| 724421 | 724431 | 12 Cell Culture Inserts + 12 Well Plate | 1.0 | Opaque | 12 | 48 |
| 725421 | 725431 | 12 Cell Culture Inserts + 24 Well Plate | 1.0 | Opaque | 12 | 48 |
| 723321 | 723331 | 6 Cell Culture Inserts + 6 Well Plate | 8.0 | | 6 | 24 |
| 724321 | 724331 | 12 Cell Culture Inserts + 12 Well Plate | 8.0 | | 12 | 48 |
| 724321 | 724331 | 12 Cell Culture Inserts + 24 Well Plate | 8.0 | | 12 | 48 |
Attention：
• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.
Guidelines for us：
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
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