RT-Bst LAMP MasterMix (with SYTO-9)
[Product Code]
210701
[Specification]
50 reactions
[Introduction]
Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique widely used in the detection of target nucleic acid. The kit provides a simple solution for LAMP and RT-LAMP assays for DNA and RNA detection. LAMP and RT-LAMP are common isothermal amplification techniques, which use LAMP-specific primers (provided by user) and strand displacement polymerase to rapidly detect target nucleic acid. The kit contains 2.5×LAMP Mix and RT-LAMP enzyme Mix, in which the 2.5×LAMP Mix contains reaction buffer, fluorescent dye, Mg2+, dNTPs, etc., and is used together with fluorescence quantitative PCR instrument or thermostatic fluorescence detector. When used, only primers and templates need to be added for nucleic acid isothermal amplification.
Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.
[Contents]
| Product composition | 50 Reactions |
| 2.5×LAMP Mix | 500 μL |
| RT-LAMP enzyme Mix | 50 μL |
| Instruction For Use | 1 pcs |
[Shipping and Storage Conditions]
The kit needs to be transported frozen and the components of the kit should be stored
away from light under -18°C.
Period of validity: 12 months.
Manufacturing date: see label.
Expiration Date: see label.
[Protocol]
1. The components were dissolved at room temperature and centrifuged briefly at 6000 RPM for 30 s. After mixing, they were set aside at 2-8°C.
2. Prepare LAMP reaction mixture in Centrifuge Tube as shown below (the whole process should be carried out in ice box):
| Component | Volume (μL) |
| 2.5×LAMP Mix | 10 |
| RT-LAMP enzyme Mix | 1 |
| 10xLAMP Primer Mix | 2.5 |
| Nucleasee free ddH2O | Add water to a volume of 20 μL |
Company Introduction
Found in 2009,Wuxi NEST Biotechnology is a worldwide manufacturer of plastic laboratory products. State-of-the-art manufacturing equipment is used on an expansive manufacturing site based in Wuxi, China. Decades of plastic-injection molding experience allows us to maintain the highest quality and performance standards expected by the hospitals, research institutions and indusrial/clinical laboraories that we serve, NEST is your best choice for laboratory consumables.
NEST has launched over 200 plastic consumable products for cell culture, molecular bislogy, immunoassays, liquid handling and storage. With stricty controlled ISO 8 grade clean rooms, exacting manufacturing standards and rigid QA procedures, the NEST brand enjoys an excellent reputation nationwide and abroad. NEST products have been exported to North America, Europe, Japan, Korea, India and other countries.
NEST is committed to innovation. We continue to research and develop new platic consumables suitable for life sciences research so that we can provide more cost-effective solutions and cutting-edge laboratory consumables for our end-users.
NEST has continually positioned itself as a brand for high end laboratory consumables.
Our Services & Strength
NEST boasts of a high power laminar flow clean room meeting the ISO 8 grade with a floor space of 2,000 square meters. These facilities are equipped with a central feeding system, high precision injection machinery from Japan, robot arms with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment machines from Belgium, Germany and USA. Inner room moisture content and temperature are adjustable. In 2013, NEST installed a Rhodotron TT 200 from Belgium IBA, a world leader in the E-Beam sterilization industry. NEST completes a full process chain from the sourcing of the raw materials to the in-house sterilizationof the final products ready for delivery to clients.
NEST’s manufacturing site has been certified with an ISO 9001:2008 management system. Our sterilization procedures meet the ISO 11137:2006 and ISO 13485 standards.
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