Fast Multiplex PCR Kit
[Product Code]
210201
[Specification]
50 reactions/kit, 6 kits/case
[Introduction]
The kit is suitable for real-time PCR analysis of DNA sample based on TaqMan probe. TaqMan qPCR is characterized by high sensitivity and specificity. The
universal nucleic amplification kit can be used for single or multiplex probe fluorescence quantitative PCR detection.
Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.
[Contents]
| Product composition | 50 Reactions |
| 2.5×qPCR Mix | 500 μL |
| Taq enzyme | 25 μL |
| Instruction For Use | 1 pcs |
[Shipping and Storage Conditions]
Period of validity: 12 months.
Manufacturing Date: see label.
Expiration Date: see label.
[Precautions]
1. 2.5x qPCR Mix should be completely dissolved before use and can be gently mixed upside down and briefly centrifuged for later use. If the reagent is not well
mixed, its performance will be degraded. Please do not use vortex oscillators for mixing, try to avoid foam.
2. Do not repeatedly freeze and thaw the reagent. It is recommended that the freeze and thaw times not exceed 3 times. After dissolving, use it as soon as
possible, within 30 minutes is recommended.
3. The DNA polymerase contained in the reagent has 5`-3` polymerase and 5`-3` exonuclease activities; has no 3`-5` exonuclease activity and no proofreading
function.
4. Taq enzyme should be centrifuged briefly before use and put back to -20°C as soon as possible after use.
5. In 25 μL reaction system, the amount of DNA template should not exceed 20% of the final volume of PCR system.
6. This reagent does not contain ROX reference dye.
7. Do not mix different batches of kit components.
8. The reaction program is only an example, please adjust according to the actual situation.
[Protocol]
Establishment of Real-Time PCR Reaction System
1. Thaw template DNA, primer, probe, dNTP Mix, 2.5×qPCR Mix, Taq enzyme, and nuclease-free water. Vortex mixing of each solution except Taq
enzyme, centrifuge briefly to collect residual liquid on tube wall, and each solution are placed on ice.
2. Prepare a fresh master mix on ice according to the table below. Centrifuge briefly to collect residual liquid on tube wall and place on ice.
Company Introduction
Found in 2009,Wuxi NEST Biotechnology is a worldwide manufacturer of plastic laboratory products. State-of-the-art manufacturing equipment is used on an expansive manufacturing site based in Wuxi, China. Decades of plastic-injection molding experience allows us to maintain the highest quality and performance standards expected by the hospitals, research institutions and indusrial/clinical laboraories that we serve, NEST is your best choice for laboratory consumables.
NEST has launched over 200 plastic consumable products for cell culture, molecular bislogy, immunoassays, liquid handling and storage. With stricty controlled ISO 8 grade clean rooms, exacting manufacturing standards and rigid QA procedures, the NEST brand enjoys an excellent reputation nationwide and abroad. NEST products have been exported to North America, Europe, Japan, Korea, India and other countries.
NEST is committed to innovation. We continue to research and develop new platic consumables suitable for life sciences research so that we can provide more cost-effective solutions and cutting-edge laboratory consumables for our end-users.
NEST has continually positioned itself as a brand for high end laboratory consumables.
Our Services & Strength
NEST boasts of a high power laminar flow clean room meeting the ISO 8 grade with a floor space of 2,000 square meters. These facilities are equipped with a central feeding system, high precision injection machinery from Japan, robot arms with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment machines from Belgium, Germany and USA. Inner room moisture content and temperature are adjustable. In 2013, NEST installed a Rhodotron TT 200 from Belgium IBA, a world leader in the E-Beam sterilization industry. NEST completes a full process chain from the sourcing of the raw materials to the in-house sterilizationof the final products ready for delivery to clients.
NEST’s manufacturing site has been certified with an ISO 9001:2008 management system. Our sterilization procedures meet the ISO 11137:2006 and ISO 13485 standards.
our team
Certifications
Customer Photos
Contact Now
