Fast Multiplex RT-PCR Kit
[Product Code]
210101
[Specification]
50 reactions/ kit, 4 kits/ case
[Introduction]
The Kit provides a convenient format for highly sensitive and specific RT-PCR using any RNA. The kit contains specific reagents for multiplex RT-qPCR using the probe method (TaqMan, etc.). It is easy to operate and does not need to open the lid during the reaction process, avoiding cross-contamination between samples and improving the detection sensitivity. This kit employs high-quality reverse transcriptase and hot-start Taq DNA polymerases, as well as our company's optimized reaction system to greatly improve the amplification performance and specificity of the reaction and it also has high amplification uniformity to effectively complete multiplex RT-qPCR. Multi RT Enzyme Mix contains both reverse transcriptase and Taq enzymes. It can be used for multiplex detection of DNA/RNA molecules and other fields.
Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.
[Contents]
| Product Composition | 50 Reactions |
| 2.5×qPCR Mix | 500 μL |
| Multi RT enzyme Mix | 50 μL |
| Instruction For Use | 1 pcs |
[Shipping and Storage Conditions]
The kit needs to be transported frozen and the components of the kit should be stored away from light under -18°C
Period of validity: 12 months.
Manufacturing Date: see label.
Expiration Date: see label.
[Precautions]
1. RNA samples should avoid RNase contamination. It is recommended to use special utensils, reagents and sterile water for RNA experiments.
2. Do not repeatedly freeze and thaw the reagent. It is recommended that the freeze and thaw times not exceed 3 times. After dissolving, use it as soon as possible, within 30 minutes is recommended.
3. Fast hot start at 95°C or 94°C for 2~5 mins should be employed.
4. The DNA polymerase contained in this reagent has 5`-3` polymerase and 5`-3` exonuclease activities; has no 3`-5` exonuclease activity and no proofreading function.
5. Multi RT enzyme Mix should be centrifuged briefly before use and should be put back to -20°C as soon as possible after use.
6. This kit must use specific primers.
7. This reagent does not contain ROX reference dye.
8. Do not mix different batches of kit components.
9. The reaction program is only an example, please adjust according to the actual situation.
Company Introduction
Found in 2009,Wuxi NEST Biotechnology is a worldwide manufacturer of plastic laboratory products. State-of-the-art manufacturing equipment is used on an expansive manufacturing site based in Wuxi, China. Decades of plastic-injection molding experience allows us to maintain the highest quality and performance standards expected by the hospitals, research institutions and indusrial/clinical laboraories that we serve, NEST is your best choice for laboratory consumables.
NEST has launched over 200 plastic consumable products for cell culture, molecular bislogy, immunoassays, liquid handling and storage. With stricty controlled ISO 8 grade clean rooms, exacting manufacturing standards and rigid QA procedures, the NEST brand enjoys an excellent reputation nationwide and abroad. NEST products have been exported to North America, Europe, Japan, Korea, India and other countries.
NEST is committed to innovation. We continue to research and develop new platic consumables suitable for life sciences research so that we can provide more cost-effective solutions and cutting-edge laboratory consumables for our end-users.
NEST has continually positioned itself as a brand for high end laboratory consumables.
Our Services & Strength
NEST boasts of a high power laminar flow clean room meeting the ISO 8 grade with a floor space of 2,000 square meters. These facilities are equipped with a central feeding system, high precision injection machinery from Japan, robot arms with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment machines from Belgium, Germany and USA. Inner room moisture content and temperature are adjustable. In 2013, NEST installed a Rhodotron TT 200 from Belgium IBA, a world leader in the E-Beam sterilization industry. NEST completes a full process chain from the sourcing of the raw materials to the in-house sterilizationof the final products ready for delivery to clients.
NEST’s manufacturing site has been certified with an ISO 9001:2008 management system. Our sterilization procedures meet the ISO 11137:2006 and ISO 13485 standards.
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