Introduction
GelNest™ Matrix is derived from mouse tumor tissue and contains extracellular matrix components such as laminin, type IV collagen, heparan sulfate proteoglycans, and more. These components support cell adhesion, differentiation, and proliferation, providing signals for these processes. Additionally, they simulate the characteristics of the basement membrane in the physiological environment, enhancing the success rate and effectiveness of cell culture.
In addition to the matrix components, GelNest™ Matrix is rich in various growth factors. These growth factors promote cell differentiation, proliferation, and migration, mimicking cellular signaling pathways and interactions in the physiological environment. GelNest™ Matrix has a wide range of applications, particularly in tissue engineering, cell culture, and research. It can be used for organoid culture, stem cell differetiation, angiogenesis, migration or invasion assays, and in vivo tumor studies.
Application
1. 2D Culture Coating
Promotes cell growth and provides a favorable environment for attachmentand proliferation.
2. Cell Migration and Tumor Invasion Experiments
Used in combination with NEST Cell Culture Insert to provide a more realistic cellular growth environment.
3. Organoid Culture
Provides a 3D cell growth environment that is physiologically relevant for studying organ development and function
4. In Vitro/In Vivo Blood Vessel Formation
Provides a 3D cell growth environment that is physiologically/pathologically relevant for studying neovascularization and maturation.
5. Stem Cell Differentiation
Used for studying stem cell differentiation and tissue regeneration.
6. In Vivo Tumor Formation Experiment
Used for in vivo tumor formation experiments in mice to study tumor growth and treatment.
Key Features
1. Protein Concentration
The protein concentration is guaranteed to be between 8~20mg/mL.
2. Stable Performance
The gel formation performance remains stable.
3. High Safety Performamce
There are no LDEV (Lactate Dehydrogenase Elevated Virus), bacteria, or mycoplasma present.
4. Endotoxin Level
The endotoxin level is <10EU/mL.
5. Cell Culture
Tests for organ-like structures and stem cell culture have been successfully performed.
6. Experimental Testing
Tests for angiogenesis, tumor invasion, and tumor formation have been successfully conducted.
GelNest™ Matrix Selection Guide
| Product | Growth Factor | Phenol Red | Recommended Applications | /Pack | /Case | Cat.NO |
| GelNest™ Matrix | Normal | Yes | General 2D, 3D cell culture | 5 mL | 1 | 211212 |
GelNest™ Matrix, without Phenol Red
| Normal
| No
| Requires colorimetric identification (such as fluorescence) or sensitivity to steroids | 5 mL | 1 | 211222 |
GelNest™ Matrix, low growth factor
| Low
| Yes
| 2D, 3D cell culture with higher accuracy
| 5 mL | 1 | 211232 |
GelNest™ Matrix, low growth factor, without Phenol Red
| Low
| No
| 2D, 3D cell culture with higher accuracy requirements for matrix components and requiresrequirements for matrix components and requires
| 5 mL | 1 | 211242 |
GelNest™ Matrix, high concentration
| Normal
| Yes
| colorimetric identification or sensitivity to steroids
| 5 mL | 1 | 211252 |
| GelNest™ Matrix, high concentration, without Phenol Red | Normal
| No
| In vivo tumor formation, thrombosis test, angiogenesis experiment, general cell culture, etc. | 5 mL | 1 | 211262 |
GelNest™ Matrix, for stem cells
| Low
| Yes
| hESC stem cell culture
| 5 mL | 1 | 211272 |
| GelNest™ Matrix, for organ-like structures, without Phenol Red | Low
| No
| Organoid culture and differentiation
| 5 mL | 1 | 211282 |
Storage and Operation Instructions
To store GelNest™ Matrix, it is recommended to keep it in a -20°C freezer until ready for use. Prior to initial use, thaw the gel and divide it into single-use portions. Store these portions in a -80°C freezer for a maximum of 2 years. Please note that GelNest™ Matrix Gel should not be stored in a frost-free freezer.
GelNest™ Matrix is in a liquid state at 4°C and transitions into a gel state at 37°C. It starts to solidify into a gel when the temperature exceeds 10°C. For optimal performance, it is advised to pre-chill the pipette tips and conduct the procedures on ice.
In addition to GelNest™ Matrix, Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.
Contact Now
