NEST frost-resistant BioFactory: unleashing your imagination for cell culture
About Vaccine Development
Process development is an essential stage in vaccine development that cannot be overlooked. It serves as the fundamental basis for the subsequent production, which encompasses crucial steps such as cell culture, fermentation, and various purification techniques. During the cell culture stage, the desired vaccine product is retained within the cells and does not naturally exit. Therefore, specific measures must be implemented to disrupt the cells and liberate the product into the surrounding liquid for subsequent purification procedures, which is called cell lysis.
About Cell Lysis Method
Chemical and enzymatic lysis methods, such as SDS and lysozyme treatment, are usually gentler. Mechanical lysis, on the other hand, is a common method that produces cells of better uniformity through repeated freeze-thaw cycles. Compared to chemical lysis, the repeated freeze-thaw method is more efficient. To implement this method, cells need to be frozen below -20°C, thawed at room temperature, and this process is repeated several times. The principle behind this method is that ice formation within the cells and increased salt concentration in the remaining cell fluid can cause swelling and lead to cell structure fragmentation. In fact, the secret to successful lysis lies in the cryopreservation capacity of containers.
High Demands for Containers