New upgrade! NEST escort your research!
The new version of NEST 384 white cell culture plate and 96 well white cell culture plate has been newly launched, which can be widely used in experiments of cell culture, absorptivity experiment, fluorescence, chemiluminescence and scintillationto, etc. NEST is devoted to inventing more light-resistant and more reliable experimental consumables to efficiently avoid optical interference and pollution.
Since the application of luciferase biosensor technology in research in 1990, the development from single luciferase detection system to double luciferase detection system has provided researchers with a more rigorous experimental approach. Compared with the single reporter gene detection system, the double luciferase reporter gene detection system consists of the "reference control" reporter gene which could eliminate the interference brought by cell growth status, cell number and transfection efficiency among different groups and achieves the function of data rectification, making the experimental results more reliable. Dual luciferase reporter gene detection system introduced the "reference control" reporter gene on the basis of single luciferase reporter gene detection system. Firefly luciferase is an intracellular protein with a size of 61KDa. In the presence of oxygen, ATP and magnesium ions, the substrate firefly fluorescein is catalyzed to oxyluciferin, which gives off yellow-Green fluorescence with a maximum luminous wavelength of 560nm.
Sea kidney luciferase is also an intracellular protein with a size of 36KDa. Oxygen is the solely condition to catalyze coelenteramide then oxidize it to a kind of high-energy product Coelenteramide, which gives off blue light with the maximum luminous wavelength of 465nm. 
As for the luminescence reaction, obvious signal interference exist between adjacent holes in the transparent plate, generating errors in the measurement results. For this reason, normally the cells were firstly cultured in the ordinary transparent cell culture plate, then were transferred to the all-white detection plate for detection after the detection reagent was added and the cells are lysed. However, this operation is complicated and easy to cause sample loss. When it comes to how to avoid signal interference while reduce sample loss, NEST`s newly upgraded cell culture plate deserves your choice. The material of the whole collection has been improved, ensuring each well independence void of adjacent light interference and more reliable. The 96-well white cell culture plate was especially launched for you diverse experimental requirement. It is NEST`s duty to provide premium experimental disposables more accurate data and convenience for clients.
